光学显微镜的使用方法英文双语对照

光学显微镜是一种用于观察微小物体的工具，通常用于生物学、医学、材料科学等领域。以下是光学显微镜的基本使用方法：

1. **准备工作**：
- 将显微镜放在平稳的表面上，并确保其稳固。
- 确保工作区域光线充足，以便观察时有足够的照明。

2. **调节光源**：
- 打开光源，通常是位于显微镜底部的光源。
- 调节光源强度，确保样本照明充分但不过亮。

3. **调节目镜**：
- 将目镜（ocular）放在显微镜上，并调节焦距，使其清晰可见。
- 目镜通常具有固定的放大倍数，如10×。

4. **调节物镜**：
- 使用可旋转的物镜（objective）来调节放大倍数。
- 通常显微镜配备有多个物镜，如4×、10×、40×、100×等，放大倍数依次增加。

5. **放置样本**：
- 将待观察的样本放置在显微镜的移动平台（stage）上。
- 通过使用移动平台上的调节螺钉来调整样本位置，使其位于光线的中心。

6. **初步调焦**：
- 使用粗调焦轮（coarse focus）将样本与物镜调至大致焦距。
- 观察样本是否清晰可见，如果模糊则继续微调直至清晰。

7. **精细调焦**：
- 使用细调焦轮（fine focus）进行微小的焦距调整，以获得更清晰的图像。

8. **观察和记录**：
- 通过目镜观察样本，可以移动样本或调节物镜以获得不同的视野和放大倍数。
- 如有需要，可以使用显微镜配备的摄像头或连接电脑来记录图像或视频。

9. **关闭和保养**：
- 关闭光源和显微镜，确保其干净整洁。
- 如有需要，定期清洁显微镜的各个部件，特别是物镜和目镜，以确保观察图像的清晰度。

以上就是基本的光学显微镜的使用方法，希望对你有所帮助！

Optical microscope is a kind of tool that is used at observing tiny object, use at the domain such as science of biology, medicine, material normally. Use a method basically be optical microscope below:

1.** prepares working ** :
- put microscope in smooth apparently, ensure its are firm.
- ensure work area light is enough, so that observe from time to tome enough illume.

2.** of ** adjustment illuminant:
- open illuminant, it is the illuminant that is located in microscope bottom normally.
- adjust illuminant intensity, ensure example illume is sufficient but bright nevertheless.

3.** moves program lens ** :
- ocular (Ocular) put on microscope, adjust focal length, make its clear and visible.
- ocular has fixed enlarge multiple normally, be like 10 × .

4.** of ** adjustment field lens:
- use the field lens that can rotate (Objective) will adjust enlarge multiple.
- normally microscope distributes stock many field lens, wait like 4 × , 10 × , 40 × , 100 × , enlarge multiple is ordinal increase.

5.** places example ** :
- set the example that needs observation the floating platform in microscope (Stage) on.
- through using the adjustment on floating platform bolt adjusts example position, make its are located in radial center.

6.** of ** preliminary focus:
- Jiao Lun of use coarse adjustment (Coarse Focus) reach example and field lens attune roughly focal length.
- observation example is visible, if blur,continue till fine tuning is clear.

7.** of ** careful focus:
- use fine focus annulus (Fine Focus) undertake tiny focal length is adjusted, in order to obtain clearer picture.

8.** observes and record ** :
- survey sample through ocular, OK and mobile example or adjust the eye shot that field lens differs with obtaining and enlarge multiple.
- if have need, can use what microscope deploys to photograph will record image or video like head or join computer.

9.** shuts and maintain ** :
- shut illuminant and microscope, ensure its are clean and neat.
- if have need, each components of fixed and clean microscope, especially field lens and ocular, observe the definition of image in order to ensure.

Above is the use method of basic optical microscope, hope to be helped somewhat to you!